Isolation and partial characterization of a new reovirus in the Chinese mitten crab, Eriocheir sinensis H Milne Edwards

A second type of freshwater crab reovirus has been isolated from Chinese mitten crab, Eriocheir sinensis H. Milne Edwards, in China; we named it E. sinensis reovirus (EsRV816). The negatively stained virion is a non‐enveloped icosahedral particle, 60 ± 5 nm in diameter. Its genome is composed of 10 dsRNA linear pieces exhibiting an electrophoretic pattern of 5/3/2. The largest segment (RNA‐1) was cloned and sequenced. The deduced amino acid sequence, corresponding to the RdRp of the virus, showed 26% identity with the RdRp of Operophtera brumata (L.) cypovirus 19 in the genus Cypovirus and 24% identity with RdRp of Nilaparvata lugens (Stal) reovirus in the genus Fijivirus. On the basis of its ultrastructure and physicochemical properties, this virus is quite different from other crab reoviruses, and particularly with another freshwater crab reovirus EsRV905, recently classified in a new genus Cardoreovirus. This virus (EsRV816) possesses all the characters of the members of the reoviridae family and could represent a new genus.     

中华绒螯蟹部分基因组文库构建和微卫星位点的筛选

利用磁珠富集和放射性杂交相结合构建了中华绒螯蟹基因组微卫星文库.并利用生物素标记的(GA)12探针进行微卫星片段的富集.将得到的片段与pGEM-T载体连接后转入DHSα大肠杆菌中,然后利用γ-32P同位素标记的(GA)12:探针进行第二次筛选.结果共获得微卫星基因组文库1 500个菌,杂交前菌落PCR检测阳性克隆率为62.5%;杂交后得到的阳性克隆为447个,占29.8%.经测序,有248个克隆含有重复次数大于5次的微卫星序列.在得到的微卫星序列中,重复单元除GA/CT外,还观察到三碱基、四碱基重复单元.根据侧翼序列没计引物164对,选择合成50对,通过优化PCR反应条件,结果有21对引物可扩增出清晰可重复的目的条带,15对具有多态性.本研究对中华绒螯蟹基因组资源的开发利用提供了相关资料.     

河蟹养殖自动作业船导航控制系统设计与测试

针对河蟹养殖过程中存在的水草清理难度大、喂料投饵不均匀、人力成本高等问题,该文设计了一种基于ARM(advanced RISC machine)和GPS/INS(global positioning system/inertial navigation system)组合导航的多功能全自动河蟹养殖作业船导航控制系统。该系统由明轮驱动船、ARM主控制器、GPS/INS组合导航装置等组成。为降低传统的基于有限目标点航道位置计算方法的复杂度并减小船体偏离航道的误差,该文提出了一种基于实时插点的航道位置计算方法,实时地解算出当前时刻的目标位置,并设计了相应的转弯及航道切换策略。针对明轮船具有非线性、大时滞、欠阻尼的运动特点,设计了基于模糊PID(proportion integration differentiation)的航向、航速双闭环运动控制算法;基于嵌入式Linux操作系统,设计了船载子系统软件,并编写了上位机监控程序对船载子系统的运行状态进行实时监控。利用河蟹养殖作业船试验平台进行了航速及自动导航试验,并对有限目标点和实时插点的航道位置计算方法的控制效果进行了对比。试验结果表明:船体速度响应较快,超调量不超过5%,稳态误差可控制在3%以内;采用有限点的航道位置计算方法时,船体在转弯与直行时偏离航道的最大误差分别为2.12和1.52m;采用实时插点的航道位置计算方法时,船体在转弯与直行时偏离航道的最大误差分别为0.36和0.09m,分别下降了83.02%和94.08%,船体的控制精度得到了全面的改善。该文可以为多功能河蟹养殖作业船的研究提供重要参考。     

Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium

The Atlantic cod (Gadus morhua) and red king crab (Paralithodes camtschaticus) processing wastes are massive and unutilized in the Murmansk region of Russia. The samples of skin-containing waste of Atlantic cod fillets production were hydrolyzed using enzyme preparations derived from red king crab hepatopancreases, porcine pancreases, and Bacillus subtilis bacteria. The activity of enzymes from crab hepatopancreases was significantly higher than the activity of enzymes derived from other sources. The optimal conditions of the hydrolysis process have been figured out. The samples of cod processing waste hydrolysate were analyzed for amino acid composition and molecular weight distribution. The samples of hydrolysate were used as core components for bacterial culture medium samples. The efficiency of the medium samples was tested for Escherichia coli growth rate; the most efficient sample had an efficiency of 95.3% of that of a commercially available medium based on fish meal. Substitution of medium components with those derived from industrial by-products is one of the ways to decrease a cost of a culture medium in biopharmaceutical drug production.     

东北稻蟹中18种微量元素含量及健康风险评价

为探讨东北地区（黑龙江省、吉林省和辽宁省）稻田养殖河蟹的微量元素含量水平和食用安全性,于2017年9月在东北主要养殖区采集稻田养殖河蟹56份,利用电感耦合等离子体质谱法（ICP-MS）检测了河蟹体内18种微量元素的含量,并采用单因子污染指数（P_i）和每周可耐受摄入量（PTWI）、目标危害商值（THQ）法分别评价了其污染程度和食用安全性。结果表明：河蟹中检出的大多数微量元素含量远高于该地区池塘养殖的淡水鱼类;吉林省河蟹体内的Cr、Cd、U等10种微量元素含量与其他两省份相比存在显著性差异（P<0.05）;所有样品的Cu、Pb、Cd和Cr含量均未超出国家食品安全标准;5种重金属（Cu、Zn、Pb、Cd和Cr）的成人每周实际摄入量（AWI）低于食品添加剂联合专家委员会（JECFA）推荐的每周可耐受摄入量;5种重金属和其他4种有害微量元素（Ba、U、Ag和Be）的人体每日摄入量（EDI）低于美国环保署（USEPA）推荐的参考剂量（RfD）。研究表明食用东北稻田养殖河蟹摄入的微量元素对食用人群没有明显的健康风险。在检测的18种微量元素中,Ba、Pb和Cd对复合污染物风险指数（HI）贡献率较高,是主要的风险元素。     

中华绒螯蟹在“蟹龙宫”饲养环境中的个体蜕壳与生长

分析"蟹龙宫"饲养中华绒螯蟹(Eriocheir sinensis)在一个蜕壳周期的蜕壳与生长情况。结果发现:"蟹龙宫"饲养中华绒螯蟹蜕壳后体质量平均增长率为33.19%,头胸甲长、头胸甲宽和体高的平均增长率为12.12%～14.97%,平均蜕壳间期为59.35 d。蜕壳前平均肥满度达60.84%,头胸甲的色度值(RGB)平均为109.17,蜕皮激素(EH)含量达14.18 IU/mL。试验河蟹在蜕壳前生长性状、EH含量和蜕壳间期长短与蜕壳后的增长率不存在显著相关性(P0.05)。在"蟹龙宫"的个体养殖系统中,中华绒螯蟹的肥满度达60%,蜕皮激素含量升至14 IU/mL时会启动蜕壳。     

淡水虾蟹养殖池塘中5种水草的营养成分比较

比较虾蟹池塘中微齿眼子菜、线叶眼子菜、菹草、空心莲子草和穗花狐尾藻5种水草的常规营养成分、总类胡萝卜素、脂肪酸和氨基酸含量,并结合5种水草的生长特性和对底泥再悬浮的抑制作用,综合评估这5种水草在淡水虾蟹养殖中的应用价值.试验结果显示:(1)菹草水分含量显著高于其他4种水草,微齿眼子菜、线叶眼子菜和空心莲子草的粗蛋白和粗...     

基于机器视觉的水下河蟹识别方法

为了探测河蟹在池塘中的数量及分布情况,为自动投饵船提供可靠的数据反馈,提出了基于机器视觉的水下河蟹识别方法。该方法通过在投饵船下方安装摄像头进行河蟹图像实时采集,针对水下光线衰减大、视野模糊等特点,采用优化的Retinex算法提高图像对比度,增强图像细节,修改基于深度卷积神经网络YOLO V3的输入输出,并采用自建的数据集对其进行训练,实现了对水下河蟹的高精度识别。实验所训练的YOLO V3模型在测试集上的平均精度均值达86. 42%,对水下河蟹识别的准确率为96. 65%,召回率为91. 30%。实验对比了多种目标检测算法,仅有YOLO V3在识别准确率和识别速率上均达到较高水平。在同一硬件平台上YOLO V3的识别速率为10. 67 f/s,优于其他算法,具有较高的实时性和应用价值。     

关于蟹-稻复合生态系统种养比例问题的探讨

稻田养蟹是一种高效立体生态农业。目前,关于种稻和蟹沟面积最佳比例问题尚无明确定论。就蟹沟面积和种稻面积比例问题展开了讨论,并得出结论,从氮、磷、钾3种元素角度考虑,其比例应分别为26.53%、13.53%和42.37%。但考虑到补充大量外源氮肥时水体分子氨会对蟹造成毒害,建议将蟹沟面积比例控制在26.53%左右。在不施或少施肥料的情况下,蟹代谢废物中的氮、磷完全能够满足水稻生长,只需补充钾肥。     

Stepwise function of natural growth for Scylla serrata in East Africa: a valuable tool for assessing growth of mud crabs in aquaculture

Predicting growth is critical in aquaculture, but models of growth are largely missing for mud crab species. Here, we present the first model of natural growth in juvenile and adult mud crabs Scylla serrata from East Africa using a stepwise growth function based on data on intermoult periods and growth at moult from field mark‐recapture, pond and laboratory studies. The results showed a sigmoid growth pattern in carapace width and suggest that S. serrata in East Africa will reach 300 g and sexual maturity ~9.9 months after settlement, and a commercial size of 500 g after 12.4 months. Analyses of the literature identified several issues with the common praxis to compare standard growth measures between aquaculture studies with different initial size or growing periods. Using the new growth function to estimate the proportional difference between modelled and obtained growth as an alternative method, we show that growth rates of S. serrata cultured in cage systems, which are dominant in East Africa, was <40% of the estimated natural growth and growth obtained in pond systems. The analysis also indicated that growth rates of S. serrata in Southeast Asia was over 50% higher compared with similar culture systems in East Africa, and that different species of mud crabs had large differences in growth rates. This study shows that growth in the present mud crab aquaculture systems in East Africa is below their expected potential. Further work is needed to identify the factors behind this observation.